yfjQ [2019-03-31 15:55:12]

minor magnesium transporter

locus: BSU08000

pI: 5.42

mw: 37.69 kDa

protein length: 319 aa

gene length: 960 bp

function: magnesium uptake

product: minor magnesium transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 871,347 → 872,306

Phenotypes of a mutant

• a ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg²⁺, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]) [Pubmed|24415722]

The protein

Structure

• [PDB|4I0U] (from Thermotoga maritima, 37% identity) [pubmed|23425532]

Expression and Regulation

Operons

• genes: [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C274 (yfjQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2272 NBRP B. subtilis, Japan]
• BKE08000 (Δ[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT
• BKK08000 (Δ[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAACCCTCCACCTG, downstream forward: _UP4_TGAGGACAGCGAGCAGCTGT
• GP2853 (''[gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]''::''tet''), available in [SW|Jörg Stülke]'s lab

References

• 24415722,23425532